Techniques To Identify And Isolate Food-Borne Pathogens In Products
By using technologies like DNA and protein arrays, researchers will gain a better understanding of how food-borne pathogens operate at the molecular level.
Food engineers will explore use of micro electromechanical systems (MEMS) and nanotechnology in the design of biosensors for detecting pathogens, spores, meat tenderness, food spoilage, and food adulteration.
Food-borne diseases include infections caused by bacteria such as Salmonella, Shigella, Campylobacter, Escherichia coli, Listeria monocytogenes, Yersinia enterocolitica, and Vibrio, and parasites such as Cryptosporidium and Cyclospora.
The National Antimicrobial Resistance Monitoring System (NARMS) is an example of a well-coordinated surveillance programme among HHS/FDA, HHS/CDC, and USDA. NARMS monitors antibiotic resistance of select food-borne pathogens isolated from clinical settings (both human and animal) and the antibiotic resistance of isolates from foods.
Molecular methods are used to detect Campylobacter in water. The purpose is to improve the quality and control of drinking water resources.
Optimizing detection method is used to find out the prevalence of Escherichia coli in livestock like pigs, and identify the risk factors for the presence of shiga-toxin genes in E. coli.
Nucleic acid hybridization is a molecular biological technique in which a labeled probe sequence is used to detect another identical or similar sequence, for example Southern hybridization and Northern hybridization. It is also used to identify Staphylococcus aureus, whereby the test time can be up to 28 hours.
Immunomagnetic separation is a rapid, sensitive and specific method to detect Salmonella spp.and Shigella in food. It is combined with real-time polymerase chain reaction (PCR) assay.
Antibody screening is used to detect the risk factors for Toxoplasma infection so as to prevent contamination of Toxoplasma in meat products, and investigate the prevalence of in pigs, cattle, etc.
By using technologies like DNA and protein arrays, researchers will gain a better understanding of how food-borne pathogens operate at the molecular level.
Food engineers will explore use of micro electromechanical systems (MEMS) and nanotechnology in the design of biosensors for detecting pathogens, spores, meat tenderness, food spoilage, and food adulteration.
Food-borne diseases include infections caused by bacteria such as Salmonella, Shigella, Campylobacter, Escherichia coli, Listeria monocytogenes, Yersinia enterocolitica, and Vibrio, and parasites such as Cryptosporidium and Cyclospora.
The National Antimicrobial Resistance Monitoring System (NARMS) is an example of a well-coordinated surveillance programme among HHS/FDA, HHS/CDC, and USDA. NARMS monitors antibiotic resistance of select food-borne pathogens isolated from clinical settings (both human and animal) and the antibiotic resistance of isolates from foods.
Molecular methods are used to detect Campylobacter in water. The purpose is to improve the quality and control of drinking water resources.
Optimizing detection method is used to find out the prevalence of Escherichia coli in livestock like pigs, and identify the risk factors for the presence of shiga-toxin genes in E. coli.
Nucleic acid hybridization is a molecular biological technique in which a labeled probe sequence is used to detect another identical or similar sequence, for example Southern hybridization and Northern hybridization. It is also used to identify Staphylococcus aureus, whereby the test time can be up to 28 hours.
Immunomagnetic separation is a rapid, sensitive and specific method to detect Salmonella spp.and Shigella in food. It is combined with real-time polymerase chain reaction (PCR) assay.
Antibody screening is used to detect the risk factors for Toxoplasma infection so as to prevent contamination of Toxoplasma in meat products, and investigate the prevalence of in pigs, cattle, etc.
References:
- http://www.socialtechnologies.com/technology/TF%20marketing%20samples/TF-2003-4%20Food%20Research%20Trends%202003%20and%20Beyond.pdf
- http://www.fao.org/docrep/meeting/008/y5871e/y5871e0n.htm
- http://www.sproutnet.com/Research/combination_of_immunomagnetic_se.htm
- http://www.biochem.northwestern.edu/holmgren/Glossary/Definitions/Def-N/nucleic_acid_hybridization.html
- http://www.mtt.fi/nkj//NKJsLivsmedelsrapport.doc
posted by meixi05 @ 2:58 AM
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